Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Structure of Cadherins01:25

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision ventral extraction of implanted mouse brains: A protocol.

STAR protocols·2026
Same author

Comparison of physician-assessed and patient-reported outcomes of using azithromycin eye drops in the treatment of bacterial blepharitis.

Japanese journal of ophthalmology·2026
Same author

Impact of Expandable Cryoballoon on Pulmonary Vein Morphology and Treatment Selection in Atrial Fibrillation Ablation.

Pacing and clinical electrophysiology : PACE·2026
Same author

Arterial spin labeling imaging of epileptic seizures with and without status epilepticus: A descriptive study.

Acta neurologica Belgica·2026
Same author

Tuning hydrogel affinity to control the release of antibodies.

Biomaterials·2026
Same author

Analysis of reconduction after cavotricuspid isthmus ablation based on computed tomography anatomy and procedural factors with emphasis on catheter stability.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2025

Related Experiment Video

Updated: Jun 24, 2026

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

E-Cadherin regulates neural stem cell self-renewal.

Phillip Karpowicz1, Sandrine Willaime-Morawek, Laurent Balenci

  • 1Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. phillip.karpowicz@utoronto.ca

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|March 27, 2009
PubMed
Summary

E-Cadherin is crucial for neural stem cell self-renewal and survival. Blocking E-Cadherin function reduces neural stem cell numbers, highlighting its role in the stem cell niche.

More Related Videos

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

Related Experiment Videos

Last Updated: Jun 24, 2026

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay
08:55

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • E-Cadherin is a cell adhesion protein involved in cell processes.
  • E-Cadherin is present in neural stem cell niches during development and in adults.

Purpose of the Study:

  • To investigate the role of E-Cadherin in neural stem cell self-renewal.
  • To determine if E-Cadherin's adhesive function is responsible for its effects on neural stem cells.

Main Methods:

  • Utilized mice with floxed E-Cadherin genes crossed with Nestin-Cre mice to study gene function in vivo and in vitro.
  • Employed adhesion-blocking antibodies targeting E-Cadherin's extracellular domains to assess functional impact.
  • Overexpressed normal and mutated E-Cadherin to evaluate the role of its intracellular binding domain.

Main Results:

  • Deletion of E-Cadherin in neural stem cells impaired self-renewal both in vivo and in vitro.
  • E-Cadherin antibodies reduced adult neural stem cell colony numbers in a dose-dependent manner, unlike P-Cadherin antibodies.
  • Overexpression of E-Cadherin, including a mutated form lacking the intracellular domain, increased clonal adult neural stem cell colonies.

Conclusions:

  • E-Cadherin-mediated adhesion is critical for neural stem cell self-renewal.
  • E-Cadherin plays a significant role in regulating the number of neural stem cells within their niche.