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Updated: Jun 24, 2026
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
Published on: October 16, 2017
Gene transfer into mammalian cells using a self-deleting avian leukemia and sarcoma virus-based vector
Caroline Torne-Celer1, Karen Moreau, Claudine Faure
1Université de Lyon, Lyon, France.
Objectives:
We have previously described an avian leukemia and sarcoma virus-based vector containing an additional att sequence in an internal position that is capable of self-deleting most of its 5' viral sequences during one cycle of replication in avian cells [Virus Res 2008;135:72-82; Arch Virol 2008;153:2233-2243]. Herein, our aim was to test the infectivity and self-deleting properties of this avian retroviral vector in human cells.
Methods:
Human Hela cells transiently expressing the cellular receptor for avian leukemia and sarcoma viruses (tva) were infected with the avian vector. Molecular analyses of thirteen clones were performed.
Results:
Data showed that more than 77% of proviruses had lost the 5' part of their genome including the selectable gene. At least 61% of these proviruses were flanked on the left by the additional att sequence and on the right by the LTR. None of the thirteen proviruses was able to express a full-length genomic RNA.
Conclusion:
This study demonstrates that the self-deleting properties of the avian vector in avian cells may be also applicable to human cells.
