Related Experiment Video
Updated: Jul 7, 2026
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
Published on: April 6, 2016
BPSS: a Nextflow pipeline for Bacterial Peptide Sequence Selection to detect protein diversity
Sylvère Bastien1,2, Pauline François1,2, Sara Moussadeq1,2
1CIRI, Center for Integrative Research in Infectious Diseases and Immunology, Université de Lyon, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, 69007, France.
Motivation:
Sequence variability can be extremely high, particularly in bacteria due to the rapid accumulation of mutations linked to their high replication rate and environmental selection pressure, which often favors diversifying selection. For most species, there are no automated, computationally efficient tools available for constructing a nonredundant database covering the allelic variability of target proteins.
Results:
We have thus developed Bacterial Peptide Sequence Selection, a Nextflow pipeline to define a minimal list of peptide sequences for detecting all variants of a protein of interest.
Availability And Implementation:
All the code and containers used are freely available on Gitlab from https://gitbio.ens-lyon.fr/ciri/stapath/bpss or on Zenodo (10.5281/zenodo.16894981) under GPLv3 open-source license and DockerHub platform from https://hub.docker.com/u/stapath.
More Related Videos
13:49Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Published on: December 6, 2017
08:31Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
