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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Supported phospholipid bilayer interaction with components found in typical room-temperature ionic liquids - a QCM-D
1New Crops and Processing Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 N. University Street, Peoria, IL 61604, USA.
Abstract:
Quartz crystal microbalance with dissipation (QCM-D) monitoring and atomic force microscopy (AFM) were combined to evaluate the defects created by an ionic liquid anion and a cation in a supported phospholipid bilayer composed of zwitterionic lipids on a silica surface. The cation 1-octyl-3-methyl imidazolium (OMIM(+)) was shown to remove lipids from the bilayer, increase the roughness to approximately 2.8 nm (~0.2 for stable supported bilayer) and possibly redeposit lipids with entrapped water. The anion bis(trifluoromethylsulfonyl)imide (Tf(2)N(-)) was found to leave distinct defects within the bilayer that had large pore-like interiors which left the surrounding bilayer intact. However, the ionic liquid 1-butyl-1-methyl pyrrolidinium bis(trifluoromethylsulfonyl)imide (BMP-Tf(2)N) formed a film over the supported bilayer. This work demonstrates, for the first time, the direct effects common components of ionic liquids have on a supported phospholipids bilayer.
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