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Updated: Jun 24, 2026

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Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
Synthesis of N-linked glycopeptides on solid support and their evaluation as protease substrates
Mallesham Bejugam1, Beatrice A Maltman, Sabine L Flitsch
1School of Chemistry, The University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh EH9 3JJ, UK.
Tetrahedron, Asymmetry
|March 28, 2009
Abstract:
A range of glycopeptides containing protease cleavage sites were synthesized on solid support using Fmoc-based solid phase glycopeptide synthesis. The immobilized peptides were studied as substrates for the proteases chymotrypsin and thermolysin. For chymotrypsin, N-glycosylation of an Asn residue at the P(2) site appears to reduce hydrolysis whereas glycosylation of the P(1) site does not appear to affect peptide hydrolysis by thermolysin.

