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Updated: Jun 24, 2026

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
Published on: July 25, 2013
The novel inhibitors of serine proteases
N Hovhannisyan1, Sh Harutyunyan, A Hovhannisyan
1Institute of Biotechnology, Yerevan, Armenia. nelliog@yahoo.com
Abstract:
Thirty optically active nonprotein alpha-amino acids and peptides based thereon have been screened for their ability to interact with bovine trypsin and proteinase K from Tritirachium album Limber, which belong to the group of serine proteases. Both structure-based drug design approach and determination of enzyme activity have been used to identify low molecular weight inhibitors of trypsin and proteinase K. Compounds have been selected that according to the docking analysis were able to interact with trypsin and proteinase K. Following the docking analysis measurement of enzymes activity (2R,3S)-beta-hydroxyleucine and (2S,3R)-beta-hydroxyleucine inhibited both enzymes activity, whereas (S)-alpha-methyl-beta-phenylalanine, (R)-alpha-methyl-beta-phenylalanine, (S)-allylglycine, (R)-allylglycine, (S)-alpha-allylalanine, (R)-alpha-allylalanine and allo-O-ethylthreonine inhibited only proteinase K; and N-formyl-(S)-methionyl-(2S,3R)-hydroxyleucine, N-formyl-(S)-methionyl-(2R,3S)-hydroxyleucine, N-formyl-(S)-methionyl-(S)-allylglycine and N-formyl-(S)-methionyl-(R)-allylglycine inhibited trypsin. It has been shown that inhibition of trypsin by (2R,3S)-beta-hydroxyleucine and N-formyl-(S)-methionyl-(2R,3S)-hydroxyleucine is of a competitive mode.
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