Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
What are Second Messengers?01:12

What are Second Messengers?

Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
What are Second Messengers?01:12

What are Second Messengers?

Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inositol biosynthesis is inversely regulated by glycolytic activity.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

Nuclear speckle dynamics are controlled by polyphosphate inhibition of CLK proteins.

Nucleic acids research·2026
Same author

Spontaneous pneumothorax-associated with genetic disorders.

Therapeutic advances in respiratory disease·2026
Same author

Restoring mitochondrial lipid homeostasis with arachidonic acid supplementation to alleviate cognitive impairment in schizophrenia patients.

Journal of advanced research·2026
Same author

Immunogenicity and safety of an Escherichia coli-produced 9-valent HPV vaccine in adolescents aged 9 to 17 compared with young women.

Science bulletin·2026
Same author

Sunflower-Inspired Zero-Energy-Consumption Thermal Switching for Dual-Mode Photochromic Smart Window.

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: Jun 24, 2026

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

Are inositol pyrophosphates signalling molecules?

Adam Burton1, Xiaowen Hu, Adolfo Saiardi

  • 1Medical Research Council, Cell Biology Unit, Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK.

Journal of Cellular Physiology
|March 28, 2009
PubMed
Summary

Inositol pyrophosphates, a class of signaling molecules, regulate cellular processes and introduce a new post-translational modification called serine-pyrophosphorylation.

More Related Videos

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Related Experiment Videos

Last Updated: Jun 24, 2026

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Area of Science:

  • Cellular signaling
  • Molecular biology
  • Biochemistry

Background:

  • Inositol pyrophosphates are a recently identified family of cytosolic molecules involved in cell signaling.
  • They possess a unique 'high energy' pyrophosphate bond and are rapidly turned over within cells.
  • Their presence in all studied eukaryotic organisms suggests a fundamental cellular role.

Purpose of the Study:

  • To explore the cell signaling roles of inositol pyrophosphates.
  • To analyze the modulation of their intracellular concentration and molecular mechanisms of action.
  • To discuss the physiological consequences of inositol pyrophosphate signaling.

Main Methods:

  • Literature review and analysis of existing research on inositol pyrophosphates.
  • Discussion of metabolic characterization and signaling pathways.
  • Examination of post-translational modifications, including serine-pyrophosphorylation.

Main Results:

  • Inositol pyrophosphates can directly phosphorylate pre-phosphorylated proteins, leading to serine-pyrophosphorylation.
  • Their cellular concentration is tightly regulated with rapid turnover.
  • Evidence suggests involvement in a wide range of cellular functions.

Conclusions:

  • Inositol pyrophosphates represent a novel signaling mechanism with significant physiological implications.
  • Further research is needed to fully elucidate their roles in specific cellular processes, especially in mammals.
  • Understanding inositol pyrophosphate signaling is crucial for deciphering fundamental cellular processes.