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Updated: Jun 24, 2026

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Published on: April 9, 2017
Identification of the asparagine synthase responsible for D-Asp amidation in the Lactococcus lactis peptidoglycan
Patrick Veiga1, Michael Erkelenz, Elvis Bernard
1INRA, UR888 Bactéries Lactiques et Pathogènes Opportunistes, F-78350 Jouy-en-Josas, France.
Abstract:
We show that in Lactococcus lactis, the gene asnH encodes the asparagine synthase involved in amidation of D-Asp present in peptidoglycan side chains and crossbridges. The level of D-Asp amidation in peptidoglycan has a strong effect on the sensitivity of bacteria to endogenous autolysins and to the cationic antimicrobials nisin and lysozyme.
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