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Published on: May 4, 2018
Expandase-like activity mediated cell-free conversion of ampicillin to cephalexin by Streptomyces sp. DRS I
1Biotechnology Division, North East Institute of Science and Technology (CSIR), Jorhat, 785006, Assam, India. debajitthakur@fastmail.fm
Abstract:
Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin-sensitive E. coli strain. Though expandase could not be identified, the presence of a hitherto unreported expandase in Streptomyces sp. DRS I is suggested.
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