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Updated: Jun 24, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
Published on: January 27, 2014
Stimulation of guanylyl cyclase-D by bicarbonate
Dagang Guo1, J Jillian Zhang, Xin-Yun Huang
1Department of Physiology, Cornell University Weill Medical College, New York, New York 10065, USA.
Abstract:
Guanylyl cyclases (GCs) catalyze the conversion of GTP to the second messenger cGMP. While some transmembrane GCs are receptors for extracellular ligands, other transmembrane GCs such as retinal-specific GC-E and GC-F are stimulated by cellular proteins. GC-D is expressed in a special group of olfactory sensory neurons. However, the direct regulatory mechanism of GC-D activity is not completely understood. Here we have demonstrated that bicarbonate directly increases the activity of purified GC-D. Bicarbonate also increases the cGMP levels in cells expressing GC-D. These results identify bicarbonate as a small molecule that regulates GC-D.
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