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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
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Assembly of multiprotein complexes that control genome function.

Christoffel Dinant1, Martijn S Luijsterburg, Thomas Höfer

  • 1Department of Pathology, Josephine Nefkens Institute, Erasmus Medical Center, Rotterdam, Netherlands.

The Journal of Cell Biology
|April 1, 2009
PubMed
Summary
This summary is machine-generated.

Live-cell imaging and mathematical modeling reveal how multiprotein complexes assemble to control genome functions. These advanced techniques uncover new properties of chromatin systems crucial for DNA repair and transcription processes.

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Area of Science:

  • Molecular Biology
  • Systems Biology
  • Genomics

Background:

  • Understanding the assembly of multiprotein complexes is crucial for deciphering genome regulation.
  • Chromatin-associated systems play vital roles in fundamental cellular processes like DNA repair and transcription.

Purpose of the Study:

  • To elucidate the assembly mechanisms of multiprotein complexes involved in genome function.
  • To investigate the emergent properties of chromatin-associated systems using advanced imaging and modeling.

Main Methods:

  • Live-cell imaging techniques to visualize molecular interactions in real-time.
  • Mathematical modeling to analyze and interpret dynamic assembly processes.
  • Studies focused on chromatin-associated systems.

Main Results:

  • Unprecedented insights into the assembly dynamics of genome-regulating multiprotein complexes.
  • Identification of novel emergent properties within chromatin-associated systems.
  • Demonstration of the utility of integrating live-cell imaging with mathematical modeling.

Conclusions:

  • The combination of live-cell imaging and mathematical modeling is a powerful approach for studying complex biological systems.
  • Emergent properties of chromatin-associated systems are key to understanding DNA repair and transcription.
  • This study advances our comprehension of genome function regulation at the molecular level.