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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 24, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
08:17

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Cryosectioning.

Simon Watkins1

  • 1University of Pittsburgh Medical School, Pittsburgh, Pennsylvania, USA.

Current Protocols in Cytometry
|April 3, 2009
PubMed
Summary
This summary is machine-generated.

This guide details frozen tissue sample preparation and sectioning for microscopy. These techniques are crucial for in situ hybridization, immunohistochemistry, and enzyme histochemistry applications.

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Flash Freezing and Cryosectioning E12.5 Mouse Brain
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Flash Freezing and Cryosectioning E12.5 Mouse Brain

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Do's and Don'ts in the Preparation of Muscle Cryosections for Histological Analysis

Published on: May 15, 2015

Related Experiment Videos

Last Updated: Jun 24, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
08:17

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Flash Freezing and Cryosectioning E12.5 Mouse Brain
28:32

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Published on: May 28, 2007

Do's and Don'ts in the Preparation of Muscle Cryosections for Histological Analysis
10:27

Do's and Don'ts in the Preparation of Muscle Cryosections for Histological Analysis

Published on: May 15, 2015

Area of Science:

  • Histology and Microscopy
  • Biotechnology

Background:

  • Frozen tissue sections are vital for various light microscopic techniques.
  • Accurate sample preparation is essential for reliable results.

Purpose of the Study:

  • To describe methods for preparing and sectioning frozen tissue samples.
  • To provide guidance for researchers using these techniques in light microscopy.

Main Methods:

  • Detailed protocols for frozen tissue sample preparation.
  • Techniques for cryosectioning of biological tissues.
  • Guidelines for handling and storing frozen tissue sections.

Main Results:

  • Successfully prepared frozen tissue sections suitable for microscopic analysis.
  • Demonstrated the utility of these sections in downstream applications.

Conclusions:

  • Standardized methods for frozen tissue sectioning enable high-quality microscopic examination.
  • These preparations are foundational for advanced techniques like immunohistochemistry and in situ hybridization.