Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

12.3K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
12.3K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.5K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.5K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

11.6K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stability and control benefits of coupled wing and tail morphing in kestrel wind-hovering flight explored using a robot replica.

Journal of the Royal Society, Interface·2026
Same author

Bridging the gap: a review of gust mitigation in birds and small uncrewed aerial vehicles.

Journal of the Royal Society, Interface·2026
Same author

Orthodontic derived microplastics impact macrophage differentiation and homeostasis.

Progress in orthodontics·2026
Same author

A wind-tunnel gust generator for soaring birds and small UAVs.

The Journal of experimental biology·2025
Same author

Elucidating the high compliance mechanism by which the urinary bladder fills under low pressures.

Scientific reports·2025
Same author

Elucidating the high compliance mechanism by which the urinary bladder fills under low pressures.

Research square·2025
Same journal

Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS.

Current protocols in cytometry·2020
Same journal

Live Imaging of the Lung.

Current protocols in cytometry·2020
Same journal

Small Particle Fluorescence and Light Scatter Calibration Using FCM<sub>PASS</sub> Software.

Current protocols in cytometry·2020
Same journal

Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.

Current protocols in cytometry·2020
Same journal

Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood.

Current protocols in cytometry·2020
Same journal

Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and COVID-19 Clinical Specimens.

Current protocols in cytometry·2020
See all related articles

Related Experiment Video

Updated: Oct 3, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

19.6K

Immunohistochemistry.

Simon Watkins1

  • 1University of Pittsburgh Medical School, Pittsburgh, Pennsylvania, USA.

Current Protocols in Cytometry
|April 3, 2009
PubMed
Summary
This summary is machine-generated.

This study details immunohistochemistry methods for protein localization in cells and tissues. It covers fluorescence and bright-field microscopy techniques, antibody selection, and troubleshooting for reliable results.

More Related Videos

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
09:11

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

17.3K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

13.0K

Related Experiment Videos

Last Updated: Oct 3, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

19.6K
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
09:11

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

17.3K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

13.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Histology

Background:

  • Immunohistochemistry is a crucial technique for visualizing protein expression within biological samples.
  • Accurate protein localization is vital for understanding cellular functions and disease mechanisms.

Purpose of the Study:

  • To provide comprehensive methods for protein labeling and detection in various biological contexts.
  • To guide researchers in selecting appropriate antibodies and detection systems for fluorescence and bright-field microscopy.
  • To offer practical troubleshooting advice for optimizing immunohistochemistry experiments.

Main Methods:

  • Detailed protocols for protein labeling in suspension cells, adherent cultures, and tissue sections.
  • Application of both fluorescence and bright-field microscopy for protein detection.
  • Guidance on selecting primary and secondary antibodies based on experimental needs.

Main Results:

  • Successful demonstration of protein localization across diverse sample types.
  • Effective application of both fluorescence and bright-field imaging modalities.
  • Identification of key factors influencing successful antibody-based protein detection.

Conclusions:

  • Immunohistochemistry is an adaptable and powerful tool for protein localization studies.
  • Careful selection of reagents and methods ensures reliable and reproducible results.
  • Troubleshooting guidelines enhance the success rate of immunohistochemistry experiments.