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Transcriptomic microarray analysis of corrinoid responsive genes in Dehalococcoides ethenogenes strain 195.

David R Johnson, Audra Nemir, Gary L Andersen

FEMS Microbiology Letters

|April 4, 2009

Summary

Dehalococcoides ethenogenes strain 195 adjusts its metabolism based on available corrinoid forms. The bacterium downregulates specific genes in response to different corrinoid supplements, indicating metabolic flexibility.

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Area of Science:

Microbiology
Environmental Science
Biochemistry

Background:

Dehalococcoides ethenogenes strain 195 uses corrinoid-dependent enzymes for reductive dehalogenation of pollutants like tetrachloroethene.
This bacterium lacks the ability to synthesize corrinoids de novo, making external sources essential for its function.

Discussion:

Whole-genome microarrays revealed differential gene expression in strain 195 when exposed to excess versus limiting cyanocobalamin.
Exposure to spent medium from a methanogenic culture (ANAS) led to distinct gene regulatory responses compared to cyanocobalamin alone.
Specific genes regulated by putative cobalamin riboswitches were identified, including those involved in lower ligand base synthesis.

Key Insights:

ANAS spent medium likely contains corrinoid forms other than cyanocobalamin, triggering unique metabolic adjustments in strain 195.
Strain 195 exhibits a regulated response to different corrinoid forms, downregulating genes for cobalamin transport when cyanocobalamin is in excess.
Metabolic regulation in strain 195 is influenced by the type and availability of external corrinoids.

Outlook:

Further research can elucidate the specific corrinoid compounds present in ANAS spent medium.
Understanding these regulatory mechanisms can inform strategies for bioremediation of chlorinated ethenes.
Investigating the structural and functional diversity of corrinoid uptake and utilization in related microorganisms.