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Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus

Marcus Resch1, Heide Marie Roth, Mathias Kottmair

  • 1Lehrstuhl für Biotechnik, Department of Biology, Friederich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|April 4, 2009
PubMed

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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...