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Related Experiment Video

Updated: Jun 24, 2026

Impact of Common Fruit Juices and Toothbrushing on the Discoloration of Primary Teeth

Impact of Common Fruit Juices and Toothbrushing on the Discoloration of Primary Teeth

Published on: March 27, 2026

Root dentin body moisture changes caused by ascorbic acid.

Takashi Komabayashi¹, Chul Ahn, Song Zhang

¹Department of Endodontics, Texas A&M Health Science Center Baylor College of Dentistry, 3302 Gaston Avenue, Dallas, TX 75246, USA. ICD38719@nifty.com

Journal of Dentistry

|April 7, 2009

Summary

This summary is machine-generated.

Ascorbic acid significantly increased root dentin moisture compared to distilled water. However, soaking root dentin in either solution for longer than three days did not further enhance moisture absorption.

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Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Published on: April 26, 2024

Area of Science:

Biomaterials Science
Dental Materials Science
Dentin Hydration Studies

Background:

Root dentin's moisture content is crucial for its mechanical properties and interaction with dental materials.
Understanding how different solutions affect dentin moisture is important for endodontic and restorative procedures.
Ascorbic acid is a biocompatible agent with potential applications in endodontics.

Purpose of the Study:

To quantitatively assess the in vitro root dentin moisture (DM) absorption when immersed in 10% ascorbic acid solution versus distilled water.
To determine the time-dependent changes in root dentin moisture over a 14-day period.
To compare the efficacy of ascorbic acid in increasing dentin moisture relative to a neutral control.

Main Methods:

Forty-four human mandibular incisors were sectioned to obtain root dentin samples.
Samples were divided into two groups: 10% ascorbic acid (Group A) and distilled water (Group B).
Dentin moisture was measured at baseline and on days 1, 3, 7, and 14 using a digital moisture meter, with generalized estimating equation analysis accounting for baseline differences.

Main Results:

Group A (ascorbic acid) exhibited significantly higher baseline root dentin moisture than Group B (distilled water) (p=0.006).
After 14 days, Group A showed a mean increase in DM of 4.35+/-0.26, significantly greater than Group B.
Both groups demonstrated that soaking root dentin beyond 3 days did not lead to a further significant increase in moisture content.

Conclusions:

10% ascorbic acid solution significantly enhances root dentin moisture absorption in vitro compared to distilled water.
The rate of moisture absorption in root dentin appears to plateau after approximately 3 days of immersion in both ascorbic acid and distilled water.
These findings have implications for understanding dentin-root canal interactions and the potential use of ascorbic acid in endodontic treatments.