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Updated: Jun 24, 2026

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms
Published on: January 12, 2024
Ancylobacter dichloromethanicus sp. nov.--a new aerobic facultatively methylotrophic bacterium utilizing
Julia Firsova1, Nina Doronina, Elke Lang
1Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Abstract:
A novel aerobic facultative methylotroph was isolated from contaminated soil. The organism (strain DM16) is a Gram-negative asporogenous non-motile curved rod multiplying by binary fission. Cells are neutrophilic and mesophilic. This strain utilized dichloromethane, methanol, formate and formaldehyde along with a variety of polycarbon compounds. Strain DM16 employs the ribulosebisphosphate pathway for C1 assimilation. The DNA G+C content is 64.5 mol%. The major ubiquinone is Q-10. The dominant cellular fatty acids are 18:1omega7c (58.6%), cyclo-19:0omega8c (34.8%) and 16:0 (3.2%). Sequencing of the 16S rRNA gene and DNA-DNA hybridization experiments clearly indicated that this methylotroph should be classified as a new species within genus Ancylobacter--Ancylobacter dichloromethanicus sp. nov. with the type strain DM16T (DSM 21507T = VKM B-2484T).
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