Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Silicotungstate-Intercalated FeCoNiMn Layered Hydroxide with an Interlocked Structure for Enhanced Electrocatalytic Oxygen Evolution Reaction.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Enhanced electroactivity and diffusion by cobalt atomic clusters impregnated in biomass-derived porous carbon for room temperature Na-S batteries.

Journal of colloid and interface science·2025
Same author

Light-induced coupling of bioelectricity generation and nitrogen assimilation in algal cathode microbial fuel cells.

Bioresource technology·2025
Same author

Facile engineering bifunctional rhodium‑nickel metallene catalyst for urea-assisted hydrogen production.

Journal of colloid and interface science·2025
Same author

Copper single-atom-based flexible aptamer biochip for simultaneous monitoring of bladder cancer-related bacteria.

Mikrochimica acta·2025
Same author

Potential-driven reaction order transitions of water oxidation on hematite photoanodes.

Nanoscale horizons·2025

Related Experiment Video

Updated: Jun 24, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

High-performance UV-curable epoxy resin-based microarray and microfluidic immunoassay devices.

Ling Yu1, Yingshuai Liu, Ye Gan

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore.

Biosensors & Bioelectronics
|April 7, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed an economical method to create sensitive 3D immunoassay devices using UV-curable epoxy resin. These devices enable rapid detection of diseases, aiding point-of-care diagnostics and high-throughput screening.

More Related Videos

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

Related Experiment Videos

Last Updated: Jun 24, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Immunoassay devices are crucial for disease diagnosis.
  • Existing fabrication methods can be complex and costly.
  • Need for sensitive, portable, and affordable diagnostic tools.

Purpose of the Study:

  • To develop a simple, economic method for fabricating 3D immunoassay devices.
  • To create epoxy resin-based microarray and microfluidic systems with functional epoxide groups.
  • To optimize the performance of these devices for sensitive protein detection.

Main Methods:

  • Fabrication of 3D patterned structures using UV-curable resin.
  • Introduction of epoxide groups for protein immobilization.
  • Optimization of printing buffer, probe concentration, and immobilization time for microarrays.
  • Development of microfluidic devices for immunoassay detection.

Main Results:

  • Achieved a 5-order dynamic range and a limit of detection (LOD) of 10 pg mL(-1) for immunoglobulin G (IgG) in microarrays.
  • Demonstrated a LOD of 100 pg mL(-1) for IL-5 detection in microfluidic devices.
  • Enabled colorimetric protein detection by the naked eye.

Conclusions:

  • The developed method offers a simple and inexpensive approach for fabricating sensitive immunoassay devices.
  • The 3D microfluidic systems show potential for portable, point-of-care diagnostics.
  • This technology supports high-throughput screening for infectious diseases.