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Related Concept Videos

Phagocytosis00:41

Phagocytosis

Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis (“cellular eating”) is one of three major types of endocytosis. Cells use phagocytosis to take in large objects—such as other cells (or their debris), bacteria, and even viruses.The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). It is perhaps unsurprising, that many...
Phagocytosis00:41

Phagocytosis

Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis ("cellular eating") is one of three major types of endocytosis. Cells use phagocytosis to take in large objects, such as other cells (or their debris), bacteria, and even viruses.
The objective of phagocytosis is often destruction. Cells use phagocytosis to eliminate unwelcome visitors, like pathogens (e.g., viruses and bacteria). Many immune system cells, including...

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Related Experiment Video

Updated: Jun 24, 2026

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
10:07

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

Published on: August 26, 2016

Large scale phagosome preparation.

Adrien F Vinet1, Albert Descoteaux

  • 1INRS-Institut Armand Frappier, Laval, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2009
PubMed
Summary
This summary is machine-generated.

Phagocytosis is key for macrophages to fight infections. This study details a method using latex beads to isolate phagosomes, aiding research into how microbes evade immune cells.

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Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

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Related Experiment Videos

Last Updated: Jun 24, 2026

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
10:07

"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

Published on: August 26, 2016

Study of Phagolysosome Biogenesis in Live Macrophages
08:06

Study of Phagolysosome Biogenesis in Live Macrophages

Published on: March 10, 2014

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
12:42

Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

Published on: April 5, 2017

Area of Science:

  • Cell Biology
  • Immunology

Background:

  • Phagocytosis is a crucial cellular process where macrophages engulf and eliminate pathogens and cellular debris.
  • Macrophages are vital in resolving microbial infections, but pathogens often evade their defenses.
  • Understanding phagosome formation and maturation is essential for developing strategies against microbial evasion tactics.

Purpose of the Study:

  • To outline a method for large-scale isolation of highly pure phagosome preparations.
  • To facilitate a deeper understanding of phagosome biology and host-pathogen interactions.
  • To provide a valuable tool for studying microbial strategies that circumvent macrophage defenses.

Main Methods:

  • Utilizing latex beads to mimic host-pathogen interactions during phagocytosis.
  • Developing a large-scale approach for phagosome purification.
  • Combining isolation techniques with functional assays.

Main Results:

  • The proposed method allows for the isolation of phagosomes with high purity.
  • Latex bead-based phagosome isolation offers advantages over pathogen-containing phagosome purification.
  • This system effectively mimics key aspects of host-pathogen interactions.

Conclusions:

  • The described approach is optimal for advancing knowledge of phagosome biology.
  • This method enables large-scale study of phagosome functional properties.
  • It offers a promising avenue for developing novel strategies against microbial infections.