Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Introduction: cell-based assays for high-throughput screening.

W Frank An1, Nicola J Tolliday

  • 1Chemical Biology Platform, Broad Institute of Harvard and MIT, Cambridge, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of Selective Inhibitors of Endoplasmic Reticulum Aminopeptidase 1.

Journal of medicinal chemistry·2019
Same author

Retraction Note: Selective killing of cancer cells by a small molecule targeting the stress response to ROS.

Nature·2018
Same author

Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia.

Cell·2016
Same author

Inhibitors of Glycogen Synthase Kinase 3 with Exquisite Kinome-Wide Selectivity and Their Functional Effects.

ACS chemical biology·2016
Same author

Targeting BRCA1- and BRCA2-deficient cells with RAD52 small molecule inhibitors.

Nucleic acids research·2016
Same author

Corrigendum: Selective killing of cancer cells by a small molecule targeting the stress response to ROS.

Nature·2015
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Cell-based assays are crucial for high-throughput screening (HTS), comprising half of all screens. This review covers their history, challenges, benefits, and experimental design for successful HTS.

Area of Science:

  • Drug Discovery
  • Biotechnology
  • Pharmacology

Background:

  • Cell-based assays constitute approximately 50% of current high-throughput screening (HTS) operations.
  • HTS is a vital method in drug discovery and biological research.

Purpose of the Study:

  • To provide a comprehensive review of cell-based HTS.
  • To summarize the history, challenges, and benefits of using cell-based assays in HTS.
  • To introduce methodologies and techniques for experimental design and execution in cell-based HTS.

Main Methods:

  • Review of historical and current trends in high-throughput screening.
  • Analysis of challenges and benefits specific to cell-based assays.
  • Introduction to strategies for assay development, primary/secondary screening, and target identification.

More Related Videos

High Content Screening in Neurodegenerative Diseases
13:32

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

Related Experiment Videos

Last Updated: Jun 24, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

High Content Screening in Neurodegenerative Diseases
13:32

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

Main Results:

  • Cell-based assays are a cornerstone of modern HTS.
  • Successful HTS requires careful experimental design and strategic implementation.
  • Key considerations include assay development, screening phases, and target identification.

Conclusions:

  • Cell-based HTS offers significant advantages but also presents unique challenges.
  • Effective experimental design is critical for maximizing the success of cell-based HTS.
  • This review serves as an introduction to the methodologies discussed in the book.