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Updated: Jun 24, 2026
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
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Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis
Patiyan Andersson1, Tegan Harris, Steven Y C Tong
1Menzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, Northern Territory 0810, Australia.
Abstract:
Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.
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