Related Experiment Video

Updated: Jun 24, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Controlling the selection stringency of phage display using a microfluidic device

Yanli Liu1, Jonathan D Adams, Kelisha Turner

  • 1Neuroscience Research Institute, University of California, Santa Barbara, CA93106, USA.

Lab on a Chip
|April 8, 2009
PubMed

Insights

Microfluidic technology precisely controls phage selection washing stringency. This enables rapid isolation of diverse peptide sequences, advancing automated directed evolution for affinity reagents.

Area of Science:

  • Biotechnology
  • Biochemistry
  • Microfluidics

Background:

  • Phage selection is a key method for discovering affinity reagents.
  • Current methods can be time-consuming and lack precise control over selection parameters.
  • Microfluidic systems offer potential for enhanced control and automation.

Purpose of the Study:

  • To investigate the impact of controlled washing stringency in microfluidics on phage selection.
  • To demonstrate the utility of a microfluidic magnetic separator (MMS) for peptide sequence isolation.
  • To explore the foundation for automated microsystems in directed evolution.

Main Methods:

  • Utilized microfluidic technology for phage selection.
  • Employed a microfluidic magnetic separator (MMS) for controlled washing.
  • Varied washing stringency to assess its effect on isolated peptide diversity.

Main Results:

  • Accurate control of washing stringency in the MMS directly impacted the diversity of isolated peptide sequences.
  • Reproducible magnetic and fluidic forces enabled controlled washing conditions.
  • Rapid convergence of selected peptide sequences was observed.

Conclusions:

  • Microfluidic control over washing stringency is critical for optimizing phage selection.
  • The developed MMS facilitates rapid and controlled isolation of peptide sequences.
  • Findings support the development of automated microsystems for in vitro directed evolution of affinity reagents.

More Related Videos

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Related Concept Videos