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Updated: Jun 24, 2026

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
[Oxidative modification of proteins, its role in pathologic states]
Oxidative modification of proteins, influenced by amino acid composition, plays a key role in various pathologies. This process generates radicals and hydroperoxides, serving as early markers of tissue damage in reactive species pathology.
Area of Science:
- Biochemistry
- Molecular Biology
- Pathology
Context:
- Oxidative modification of proteins is a complex process influenced by protein structure and amino acid composition.
- It involves the formation of radicals and long-life hydroperoxides, contributing to oxidative stress.
- Protein modification is linked to the regulation of protein synthesis and degradation via multicatalytic proteases.
Purpose:
- To review the principal mechanisms of protein oxidative modification.
- To elucidate the role of oxidative modification in various pathologies.
- To discuss the formation of radicals and hydroperoxides during oxidative stress.
Summary:
- Oxidative modification of proteins involves alterations to the polypeptide chain and amino acid residues, leading to radical formation.
- Electron-transfer reactions are crucial for generating radical centers on protein surfaces.
- Oxidative protein destruction is a reliable early indicator of tissue injury in reactive species pathology.
Impact:
- Understanding these mechanisms aids in identifying therapeutic targets for diseases associated with oxidative stress.
- This knowledge can lead to the development of novel biomarkers for early disease detection.
- The findings contribute to a deeper comprehension of cellular damage and protective mechanisms.
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