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Donor cornea bacterial contamination.

P L Farrell1, J T Fan, R E Smith

  • 1Doheny Eye Institute, Los Angeles, California.

Cornea
|September 1, 1991
PubMed
Summary
This summary is machine-generated.

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Cornea rim contamination in donor corneas was 14.1%, with common bacteria resistant to gentamicin. However, vancomycin proved effective against these resistant organisms, suggesting improved antibiotic strategies for corneal transplantation.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Transplantation Science

Background:

  • Donor cornea contamination is a potential risk in corneal transplantation.
  • Understanding the prevalence and antibiotic resistance patterns of contaminating organisms is crucial for patient safety.

Purpose of the Study:

  • To determine the incidence of culture-positive cornea rims from donor corneas.
  • To identify common contaminating organisms and their antibiotic sensitivities.
  • To inform strategies for reducing contamination and guiding perioperative antibiotic selection.

Main Methods:

  • Prospective culture of 446 consecutive donor cornea rims.
  • Identification of bacterial isolates.
  • Antimicrobial susceptibility testing, focusing on gentamicin and vancomycin resistance.

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Main Results:

  • 14.1% (63/446) of cornea rims were culture-positive.
  • Common contaminants included Streptococcus, Propionibacterium, Staphylococcus, and diptheroids.
  • Most isolates showed resistance to gentamicin, but were sensitive to vancomycin.
  • No patients who received contaminated corneas developed endophthalmitis.

Conclusions:

  • Donor cornea contamination is prevalent but may not always lead to adverse outcomes.
  • Gentamicin resistance is common among cornea rim contaminants.
  • Vancomycin is a viable alternative for treating gentamicin-resistant bacteria in this context.
  • Eye banks and surgeons should reconsider current antibiotic protocols for storage media and perioperative use.