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Related Experiment Video

Updated: Jun 24, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

Using quantum dots to visualize clathrin associations.

Y Ogunkoya¹, B M Nickel, V L Gay

¹Department of Biological Sciences, College of Sciences, Southern University and A & M College, Baton Rouge, Louisiana, USA.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission

|April 9, 2009

Summary

Quantum immune-electron microscopy reveals clathrin

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Clathrin-mediated endocytosis is crucial for cellular processes.
Coated pits and vesicles are key structures in endocytosis.
Current methods have limitations in precisely tracking cellular organelles.

Purpose of the Study:

To precisely localize clathrin and its adaptor protein AP-2 in human adrenal cortical cells.
To investigate the association of clathrin with various vesicle types.
To clarify the nature of structures identified as coated vesicles by light microscopy.

Main Methods:

Quantum immune-electron microscopy was employed.
Human adrenal cortical cell line (SW-13) was used.
Localization of clathrin and AP-2 with cellular organelles was performed.

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Last Updated: Jun 24, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

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Published on: October 20, 2014

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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

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Main Results:

Clathrin was found to associate with multiple vesicle types, including coated vesicles, pits, pentilaminar annular gap junction vesicles, and multivesicular bodies.
Quantum dot technology provided accurate and specific localization.
Some structures identified as coated vesicles by light microscopy may be membrane-bound pits.

Conclusions:

Quantum immune-electron microscopy offers high precision in localizing clathrin and AP-2.
Clathrin's role extends beyond classic endocytosis to other vesicle types.
Re-evaluation of structures identified by light microscopy may be necessary.