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Related Experiment Video

Updated: Jun 24, 2026

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Published on: May 10, 2021

Procyanidins-crosslinked heart valve matrix: anticalcification effect.

Wanyin Zhai¹, Jiang Chang, Xiqin Lü

¹Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, People's Republic of China.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials

|April 9, 2009

Summary

Procyanidins (PC) effectively inhibit calcification in bioprosthetic heart valves, improving durability. This study shows PC-crosslinked valves prevent mineral deposition and cellular calcification, offering a promising alternative to glutaraldehyde.

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Last Updated: Jun 24, 2026

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

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Published on: May 10, 2021

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Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Published on: November 20, 2017

Area of Science:

Biomaterials Science
Cardiovascular Research
Tissue Engineering

Background:

Glutaraldehyde crosslinking limits bioprosthetic heart valve durability due to calcification.
Procyanidins (PC) offer a potential alternative crosslinking agent with promising preliminary results.

Purpose of the Study:

To investigate the anticalcification efficacy of procyanidins (PC) and PC-crosslinked heart valves.
To evaluate the impact of PC on valvular matrix mineralization and cellular calcification.

Main Methods:

Decellularized porcine aortic valve leaflets were crosslinked with PC solution.
Valves were incubated in simulated body fluid to assess matrix calcification.
Valvular cells were cultured with PC to evaluate cellular anticalcification effects.

Main Results:

PC-crosslinked valves demonstrated inhibited matrix mineralization compared to non-crosslinked and glutaraldehyde-crosslinked valves.
PC significantly suppressed alkaline phosphatase activity and mineral deposition in valvular cells.
PC exhibited a dose-dependent inhibition of both matrix and cellular calcification.

Conclusions:

Procyanidins effectively inhibit bioprosthetic heart valve calcification by preventing mineral deposition and suppressing cellular osteodifferentiation.
PC-crosslinked heart valve matrix presents a viable candidate for improved durability in bioprosthetic implants.
This research highlights procyanidins as a promising strategy to enhance the longevity of artificial heart valves.