Surface plasmon resonance assay for chloramphenicol without surface regeneration

Area of Science:

• Analytical Chemistry
• Biochemistry
• Immunotechnology

Background:

• Chloramphenicol (CAP) is an antibiotic that requires sensitive detection methods.
• Existing Surface Plasmon Resonance (SPR) assays often require surface regeneration, adding complexity and time.
• Developing a rapid, sensitive, and regeneration-free SPR assay for CAP is highly desirable.

Purpose of the Study:

• To develop a novel Surface Plasmon Resonance (SPR) immunoassay for the rapid and sensitive detection of chloramphenicol (CAP).
• To achieve high sensitivity and speed without the need for sensor surface regeneration.

Main Methods:

• Synthesized a chloramphenicol-amine derivative with a polyethylene glycol chain.
• Immobilized the derivative onto a Biacore dextran surface to create a chemically modified surface.
• Utilized Surface Plasmon Resonance (SPR) to monitor antibody-CAP binding kinetics.

Main Results:

• The modified surface altered antibody-CAP binding, resulting in fast association and dissociation rates.
• Achieved a sensitive SPR immunoassay for CAP detection without requiring surface regeneration.
• Established limits of detection of 32.2 pg/ml in aqueous buffer and 42.4 pg/ml in honey-spiked samples.

Conclusions:

• A novel SPR assay provides a rapid and sensitive method for chloramphenicol detection.
• The regeneration-free approach simplifies the assay and enhances efficiency.
• The developed method demonstrates potential for accurate CAP quantification in complex matrices like honey.