Efficiency of a bienzyme sequential reaction system immobilized on polyelectrolyte multilayer-coated colloids

Area of Science:

• Biomaterials science
• Enzyme immobilization
• Colloid chemistry

Background:

• Enzyme immobilization is crucial for developing biosensors and biocatalysts.
• Multilayer films offer controlled enzyme arrangement and enhanced functionality.
• Silica microparticles provide a versatile platform for surface modification.

Purpose of the Study:

• To investigate the effect of polyelectrolyte combinations on enzyme immobilization and functionality.
• To optimize multilayer film configurations for enhanced sequential enzyme reactions.
• To analyze the reaction-diffusion kinetics of coimmobilized glucose oxidase and horseradish peroxidase.

Main Methods:

• Assembly of multilayer films with glucose oxidase (GOx) and horseradish peroxidase (HRP) on silica microparticles.
• Utilizing precomplexation with a polyvinylpyridine-based polyamine for stable enzyme adsorption.
• Quantitative analysis of sequential enzymatic reactions and kinetic parameters.

Main Results:

• Stable enzyme adsorption was achieved without compromising catalytic activity using precomplexation.
• Different polyelectrolyte combinations influenced enzyme immobilization and functionality.
• The optimized configuration, with GOx and HRP coimmobilized in the same layer, showed a 2.5-fold increase in hydrogen peroxide conversion rate.

Conclusions:

• Coimmobilization of GOx and HRP in a single layer on silica microparticles significantly enhances catalytic efficiency.
• Polyelectrolyte-mediated multilayer assembly provides a tunable platform for enzyme functionalization.
• This approach holds promise for developing advanced biocatalytic systems and biosensors.