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Updated: Jun 24, 2026

Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission
Published on: April 22, 2021
Imaging protein-protein interactions by Förster resonance energy transfer (FRET) microscopy in live cells
Joseph A Brzostowski1, Tobias Meckel1, Jiang Hong1
1NIH/NIAID-LIG Imaging Facility, Rockville, Maryland.
Abstract:
This unit describes an acceptor-sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end-point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET measurements in live cells and can be used to measure changes in FRET efficiency over time. The purpose of this unit is to provide a basic starting point for understanding and performing the sensitized emission method with a simple teaching tool for live-cell imaging. References that discuss the vagaries of acquiring and analyzing FRET between individually tagged molecules are provided.
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