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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 24, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Trends in microRNA detection.

Kyle A Cissell1, Sapna K Deo

  • 1Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46217, USA.

Analytical and Bioanalytical Chemistry
|April 16, 2009
PubMed
Summary
This summary is machine-generated.

Detecting microRNA (miRNA) expression is crucial for understanding disease. Current methods have limitations, necessitating the development of more sensitive, rapid, and in vivo applicable detection techniques.

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

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Last Updated: Jun 24, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression implicated in diseases like cancer.
  • Accurate detection of miRNA expression levels is vital for understanding their role in disease pathogenesis.
  • Northern blotting, the standard miRNA detection method, suffers from low sensitivity and time-consuming procedures.

Purpose of the Study:

  • To review current miRNA detection methods and identify existing gaps.
  • To discuss the advantages and disadvantages of solid-phase and solution-phase miRNA detection techniques.
  • To explore the future outlook for miRNA detection technologies.

Main Methods:

  • Overview of existing miRNA detection strategies, including Northern blotting, microarray technology, luminescence-based assays, and electrochemical assays.
  • Categorization of methods into solid-phase and solution-phase approaches.
  • Analysis of sensitivity, throughput, and in vivo applicability of different techniques.

Main Results:

  • Solid-phase methods offer high throughput and sensitivity but lack in vivo capability and can be time-consuming.
  • Solution-phase methods are rapid, sensitive, and suitable for in vivo applications but not for high-throughput screening.
  • Existing methods struggle to achieve multiplex, sensitive detection with single-nucleotide specificity.

Conclusions:

  • Significant advancements in miRNA detection are needed to address current limitations.
  • Future research should focus on developing standardized methods for sensitive, multiplexed, and single-nucleotide specific miRNA detection.
  • Improved detection technologies will facilitate a deeper understanding of miRNA's role in disease and therapeutic development.