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Related Experiment Video

Updated: Jan 30, 2026

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

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Published on: July 31, 2017

Optimization of Ca(v)1.2 screening with an automated planar patch clamp platform.

Bharathi Balasubramanian¹, John P Imredy, David Kim

¹Safety and Exploratory Pharmacology, Safety Assessment, Merck Research Laboratories, West Point, PA 19486, USA. bharathi_balasubramanian@merck.com

Journal of Pharmacological and Toxicological Methods

|April 16, 2009

Summary

A new assay for screening Ca(v)1.2 channel blockers was developed using PatchXpress. This method offers a robust, medium-throughput approach for assessing drug cardiac liability during lead optimization.

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Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

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Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Published on: February 7, 2012

Area of Science:

Cardiovascular Pharmacology
Ion Channel Physiology
Drug Discovery

Background:

Calcium voltage-gated channel subfamily 1.2 (Ca(v)1.2) channels are crucial for cardiac action potential.
Screening for Ca(v)1.2 blockers is vital for identifying drugs lacking cardiac liability.
Traditional fluorescence assays have limitations, and patch clamping is labor-intensive.

Purpose of the Study:

Develop a robust, medium-throughput Ca(v)1.2 screening assay using PatchXpress 7000A.
Optimize cell isolation, recording solutions, and experimental parameters for reliable screening.

Main Methods:

Utilized HEK-293 cells stably expressing human Ca(v)1.2 channels.
Optimized cell isolation by reducing trypsin exposure time and temperature.
Modified intracellular and extracellular solutions to minimize channel rundown and enhance sensitivity.

Main Results:

Shortened incubation and trypsin exposure improved Ca(v)1.2 current amplitudes.
Reduced intracellular Mg2+ and added Ca2+ significantly minimized current rundown.
The assay successfully identified and characterized structurally diverse Ca(v)1.2 antagonists and an agonist.

Conclusions:

The developed PatchXpress assay provides a reliable method for Ca(v)1.2 channel screening.
This assay is suitable for assessing compound cardiac liability during drug lead optimization.