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Updated: Jun 24, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Oxidative stability of conjugated linolenic acids
Lin Yang1, Ying Cao, Jing-Nan Chen
1College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan Province 453007, China. yanglin1819@163.com
Abstract:
Interest in conjugated linolenic acid (CLnA) and conjugated linoleic acid (CLA) as functional lipids is growing. The present study was (i) to study the oxidative stability of individual CLnA isomers and (ii) to compare the oxidative stabilities of CLnA and CLA with their corresponding nonconjugated counterparts, α-linolenic acid (LN) and linoleic acid (LA). The oxidation was carried out in air at 50 °C and monitored by the gas-liquid chromatography (GC) and the oxygen consumption test. First, it was found that CLnA was most unstable followed by CLA, LN, and LA in decreasing order. Second, analyses of silver ion high-performance liquid chromatography (Ag(+)-HPLC) demonstrated that t,t,t-CLnA isomers had greater stability than c,t,t-CLnA and c,t,c-CLnA isomers. Finally, both green tea catechins (GTCs) and butylated hydroxytoluene (BHT) were capable of preventing the CLnA oxidation, with the former being more effective than the latter.
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