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Flow-through functionalized PDMS microfluidic channels with dextran derivative for ELISAs.

Ling Yu1, Chang Ming Li, Yingshuai Liu

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Dr., Singapore637457.

Lab on a Chip
|April 17, 2009
PubMed
Summary

A new dextran-modified PDMS microfluidic ELISA device offers enhanced hydrophilicity and protein immobilization. This economical, sensitive lab-on-chip system enables rapid, portable diagnostics for infectious diseases via visual detection.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Polydimethylsiloxane (PDMS) microfluidic devices are widely used for immunoassays.
  • Improving surface properties of PDMS is crucial for enhanced protein immobilization and assay sensitivity.
  • Existing PDMS-based immunoassay devices often face limitations in sensitivity and ease of use.

Purpose of the Study:

  • To develop a dextran-modified PDMS microfluidic ELISA device with enhanced hydrophilicity and protein immobilization capabilities.
  • To evaluate the performance of the fabricated device for simultaneous detection of multiple biomarkers.
  • To demonstrate the potential for visual, point-of-care diagnostic applications.

Main Methods:

  • Fabrication of a PDMS microfluidic device.

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  • Dextran functionalization of the PDMS surface using a flow-through process.
  • Covalent immobilization of antibodies for ELISA.
  • Simultaneous colorimetric detection of Interleukin-5 (IL-5), Hepatitis B surface antigen (HBsAg), and Immunoglobulin G (IgG).

    • Main Results:

    • Achieved significant enhancement of surface hydrophilicity and efficient covalent protein immobilization.
    • Demonstrated a limit of detection of 100 pg/mL and a dynamic range of 5 orders of magnitude for biomarker detection.
    • Successfully enabled visual, colorimetric detection of proteins, indicating high sensitivity.

    Conclusions:

    • The dextran-modified PDMS microfluidic ELISA device offers superior performance compared to existing methods.
    • The device presents a cost-effective and sensitive platform for high-throughput screening of infectious diseases.
    • This technology holds promise for developing portable, visually-read microfluidic ELISA devices for point-of-care health services.