Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

d-limonene suppresses RANKL-induced osteoclast differentiation and promotes osteoblast activity in vitro.

Bioscience, biotechnology, and biochemistry·2024
Same author

Tumor Necrosis Factor Receptor-2 Signals Clear-Cell Renal Carcinoma Proliferation via Phosphorylated 4E Binding Protein-1 and Mitochondrial Gene Translation.

The American journal of pathology·2024
Same author

The Impact of LED Lighting Spectra in a Plant Factory on the Growth, Physiological Traits and Essential Oil Content of Lemon Balm (<i>Melissa officinalis</i>).

Plants (Basel, Switzerland)·2022
Same author

Clonal hematopoiesis and therapy-related myeloid neoplasms following neuroblastoma treatment.

Blood·2021
Same author

The E3 ligase HUWE1 inhibition as a therapeutic strategy to target MYC in multiple myeloma.

Oncogene·2020
Same author

Molecular signatures for CCN1, p21 and p27 in progressive mantle cell lymphoma.

Journal of cell communication and signaling·2018

Related Experiment Video

Updated: Jun 24, 2026

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

A rapid and sensitive method for measuring cell adhesion.

Wanhua Lu1, Lynn McCallum, Alexandra E Irvine

  • 1Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, UK, BT9 7BL, w.lu@qub.ac.uk.

Journal of Cell Communication and Signaling
|April 17, 2009
PubMed
Summary

The adapted CyQuant assay offers a simple, rapid, and sensitive method for measuring cell adhesion. This optimized cell adhesion assay eliminates lengthy fluorescent labeling, enabling accurate quantification of even small cell numbers.

More Related Videos

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
07:40

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
07:40

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

Area of Science:

  • Cell Biology
  • Biotechnology

Background:

  • Cell adhesion is a critical biological process.
  • Accurate measurement of cell adhesion is essential for various research applications.
  • Existing methods for quantifying cell adhesion can be labor-intensive and time-consuming.

Purpose of the Study:

  • To adapt the CyQuant assay for a simplified and efficient measurement of cell adhesion.
  • To develop a sensitive method capable of detecting small numbers of adherent cells.
  • To eliminate the need for complex fluorescent labeling protocols.

Main Methods:

  • Adaptation of the established CyQuant assay.
  • Implementation of a streamlined protocol without prior fluorescent cell labeling.
  • Utilizing the modified assay to quantify adherent cells.

Main Results:

  • The adapted CyQuant assay provides a simple, rapid, and highly reproducible method for cell adhesion measurement.
  • The assay demonstrates high sensitivity, capable of detecting over 1000 adherent cells.
  • Elimination of labor-intensive fluorescent labeling protocols was achieved.

Conclusions:

  • The modified CyQuant assay is an effective tool for sensitive and reproducible cell adhesion quantification.
  • This optimized assay simplifies experimental procedures, saving time and resources.
  • The method is suitable for studies requiring the measurement of small numbers of adherent cells.