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Updated: Jun 23, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS
Anna Galkin1, Jouni Jokela, Matti Wahlsten
1Center for Drug Research and Division of Pharmaceutical Biology, Faculty of Pharmacy, FIN-00014 University of Helsinki, Finland.
Abstract:
Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.
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