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Related Concept Videos

The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

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Assaying Proteasomal Degradation in a Cell-free System in Plants
07:43

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

The proteasomal system.

Tobias Jung1, Betül Catalgol, Tilman Grune

  • 1Institute of Biological Chemistry and Nutrition, University Hohenheim, Garbenstrasse 28, 70593 Stuttgart, Germany.

Molecular Aspects of Medicine
|April 18, 2009
PubMed
Summary
This summary is machine-generated.

The ubiquitin-proteasome system regulates crucial cellular functions, impacting everything from cell cycles to diseases like cancer and aging. This review summarizes current knowledge on this essential protein degradation pathway.

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Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
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Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

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Last Updated: Jun 23, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants
07:43

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
09:57

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

Published on: December 17, 2016

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

Area of Science:

  • Cellular Biology
  • Biochemistry

Background:

  • The proteasome and ubiquitin system are integral to cellular metabolism.
  • This system influences numerous cellular processes, including cell cycle, gene expression, and immune response.
  • Dysregulation is implicated in various pathologies such as cancer, neurodegenerative diseases, and aging.

Purpose of the Study:

  • To review the current understanding of the proteasome and ubiquitin-proteasome system.
  • To highlight the diverse cellular functions regulated by this degradation pathway.
  • To emphasize its essential role across bacteria, plants, and animals.

Main Methods:

  • Literature review of existing research on proteasome and ubiquitin system.
  • Synthesis of information on regulatory proteins and their impact on proteolysis.
  • Compilation of data on the role of ubiquitin-mediated degradation in protein half-life.

Main Results:

  • The ubiquitin-proteasome system affects nearly all aspects of the cellular metabolic network.
  • It plays a critical role in maintaining cell homeostasis through the degradation of proteins, including oxidized ones.
  • Specific regulator proteins fine-tune proteolysis for specialized cellular tasks.

Conclusions:

  • The proteasome and ubiquitin-proteasome system are fundamental to cellular function in all three kingdoms of life.
  • Precise regulation of protein half-life by this system is essential for cellular health.
  • Understanding this system is key to comprehending numerous physiological and pathological processes.