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Related Experiment Video

Updated: Jun 23, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Rolling-circle amplification: unshared advantages in miRNA detection.

Saskia Neubacher1, Christoph Arenz

  • 1Humboldt-Universität zu Berlin, Institut für Chemie, Brook-Taylor-Strasse 2, 12489 Berlin, Germany. christoph.arenz@chemie.hu-berlin.de

Chembiochem : a European Journal of Chemical Biology
|April 18, 2009
PubMed
Summary

Quantitative analysis of microRNAs (miRNAs) can revolutionize disease diagnostics. A novel rolling-circle amplification (RCA) method detects miRNAs at 10 fM concentrations, distinguishing single-nucleotide differences.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medical Diagnostics

Background:

  • Quantitative analysis of specific microRNAs (miRNAs) from biological samples holds significant potential for revolutionizing human disease diagnostics.
  • Accurate and sensitive detection of miRNAs is crucial for early disease identification and personalized medicine.

Purpose of the Study:

  • To introduce and validate a novel method for homogeneous miRNA detection using rolling-circle amplification (RCA).
  • To assess the sensitivity and specificity of the RCA-based method for miRNA quantification.

Main Methods:

  • Development of a novel miRNA detection assay utilizing rolling-circle amplification (RCA).
  • Optimization of reaction conditions, including the use of T4 RNA ligase 2 (T4 RnL2) at elevated temperatures.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Last Updated: Jun 23, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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  • Homogeneous detection of specific miRNAs in biological samples.

    • Main Results:

    • The RCA-based method achieved homogeneous detection of miRNAs at concentrations as low as 10 femtomolar (fM).
    • The use of T4 RnL2 at elevated temperatures demonstrated excellent discrimination between miRNAs differing by a single nucleotide.
    • The method shows promise for accurate miRNA quantification in complex biological matrices.

    Conclusions:

    • The developed RCA method offers a highly sensitive and specific approach for miRNA quantification.
    • This technique has the potential to significantly advance the field of molecular diagnostics for human diseases.
    • Further validation in clinical settings is warranted to fully explore its diagnostic capabilities.