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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Updated: Jun 23, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Microfluidic chips for mass spectrometry-based proteomics.

Jeonghoon Lee1, Steven A Soper, Kermit K Murray

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.

Journal of Mass Spectrometry : JMS
|April 18, 2009
PubMed
Summary
This summary is machine-generated.

Microfluidic devices streamline proteomic analysis by enabling high-throughput sample preparation and identification. This technology integrates microfluidic chips with mass spectrometry, reducing labor and analysis time for complex proteome studies.

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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

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Last Updated: Jun 23, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Proteomics

Background:

  • Proteomics involves complex, labor-intensive sample preparation steps.
  • Microfluidic devices offer solutions for handling small sample volumes and enabling parallel analysis.
  • Efficient interfacing between microfluidic chips and mass spectrometers is crucial for proteomic applications.

Purpose of the Study:

  • To provide an overview of microfluidic chip technologies for mass spectrometry-based proteome analysis.
  • To summarize approaches for integrating microfluidics with electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI).
  • To present applications of chip-based separation and digestion in proteomics.

Main Methods:

  • Coupling microfluidic devices with mass spectrometry (MS).
  • Utilizing microfluidic chips for sample cleanup, digestion, and separation.
  • Integrating microfluidic systems with ESI and MALDI ionization techniques.

Main Results:

  • Microfluidics significantly reduces sample volume requirements and labor intensity in proteomics.
  • Chip-based sample preparation enhances throughput for proteomic analyses.
  • Successful integration of microfluidic devices with MS enables efficient proteome identification.

Conclusions:

  • Microfluidic devices coupled to mass spectrometry are powerful tools for advancing proteomic analysis.
  • This integrated approach offers potential for high-throughput, automated, and miniaturized proteomic workflows.
  • Further development of microfluidic interfaces and applications will accelerate discoveries in proteomics.