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Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
Published on: August 18, 2023
Ultrahigh spacing tissue arrays for screening hybridoma by immunohistochemistry
Chaojun Song1, Fuli Wang, Liang Jin
1Department of Immunology, Fourth Military Medical University, 17 Changle West Road, Xi'an 710032, PR China.
Abstract:
We present a technology to make ultrahigh spacing tissue arrays (USTA) which facilitates screening hybridoma by immunohistochemistry, results in easier performance of staining and image analysis, minimal sample mixed-up and less bookkeeping efforts. 32 array cores are designated a 4 x 8 microarray, leaving 4 mm spacing between two adjacent array elements. After separated each array spot with PAP pen, one USTA is sufficient for testing of supernatants from 32 different hybridomas.
