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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

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Updated: Jun 23, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Development of human embryonic stem cell derivation.

Kamthorn Pruksananonda¹, Ruttachuk Rungsiwiwut, Pranee Numchaisrika

¹Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. fmedkpn@md.chula.ac.th

Journal of the Medical Association of Thailand = Chotmaihet Thangphaet

|April 21, 2009

Summary

This study successfully established human embryonic stem (hES)-like cells from normal human embryos. Mechanical isolation of inner cell mass and human fibroblasts as feeder layers were key to this breakthrough in Thailand.

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Published on: November 3, 2011

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Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

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Published on: November 3, 2011

Area of Science:

Stem Cell Biology
Developmental Biology
Reproductive Medicine

Background:

Human embryonic stem (hES) cells hold significant potential for regenerative medicine and disease modeling.
Establishing reliable methods for isolating and culturing hES cells is crucial for advancing these fields.

Purpose of the Study:

To establish human embryonic stem (hES)-like cells from human embryos.
To investigate different methods for isolating inner cell mass (ICM) and culturing hES cells.

Main Methods:

Experimental study involving in vitro culture of human embryos to the blastocyst stage.
Comparison of four ICM isolation methods: immunosurgery, mechanical isolation, laser-assisted, and whole blastocyst culture.
Culturing of cells on mouse or human fibroblast feeder layers and characterization using morphology, Oct-4 immunostaining, and alkaline phosphatase (AP) activity.

Main Results:

While abnormal fertilization embryos yielded ICM outgrowths, no hES-like cells were established.
Normal fertilization embryos showed successful blastocyst attachment and ICM outgrowth formation.
One hES-like cell line was successfully established using mechanical isolation of ICMs and human adult skin fibroblasts, exhibiting typical hES cell characteristics and differentiation potential.

Conclusions:

This study reports the first successful isolation of hES-like cells in Thailand.
Mechanical isolation of ICMs combined with human fibroblast feeder layers is an effective method for establishing hES-like cell lines.
The established hES-like cells demonstrate pluripotency, forming embryoid bodies and differentiating into neural-like cells.