Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

Glucose Transporters

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:

Secondary Active Transport

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...

Secondary Active Transport

Secondary Active Transport

One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...

Cotranslational Protein Translocation

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...

Glucose Absorption Into the Small Intestine

Glucose Absorption Into the Small Intestine

Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and 'exit' via the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The plant energy management machinery: an essential hub for stress resilience and developmental dynamics with great potential for crop improvement.

Journal of experimental botany·2026

Same author

Subunit 3 of the SUPERKILLER complex mediates microRNA172-directed cleavage of Nodule Number Control 1 in Medicago truncatula.

Plant physiology·2025

Same author

Ribosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complex.

Nature communications·2025

Same author

Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum.

Plant methods·2024

Same author

Ten year real world experience with ultrafiltration for the management of acute decompensated heart failure.

American heart journal plus : cardiology research and practice·2024

Same author

S<sub>1</sub> basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs.

Proceedings of the National Academy of Sciences of the United States of America·2024

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

Sucrose-mediated translational control.

Maureen Hummel¹, Fatima Rahmani, Sjef Smeekens

¹Molecular Plant Physiology, Utrecht University, Utrecht, The Netherlands.

Annals of Botany

|April 21, 2009

Summary

Plants use translational control to manage gene expression during stress and low energy. Sucrose signaling, via bZIP11 and its SC-peptide, regulates translation and ribosomal biogenesis for optimal growth.

Area of Science:

Plant molecular biology
Metabolic signaling
Gene expression regulation

Background:

Environmental factors significantly influence plant gene expression and metabolite levels.
Metabolite concentration changes impact gene expression at transcriptional and post-transcriptional levels.

Purpose of the Study:

To investigate the role of sucrose signaling in translational control of S1 class basic leucine zipper transcription factor (bZIP) genes.
To explore the regulation of ribosomal biogenesis in response to metabolic changes and stress.

Main Methods:

Analysis of upstream open reading frames (uORFs) in bZIP genes.
Identification of the Sucrose Control peptide (SC-peptide) and its function.
Examination of the interaction between bZIP11, SnRK1 kinases, and ribosomal biogenesis regulators like TOR.

More Related Videos

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

Related Experiment Videos

Last Updated: Jun 23, 2026

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

Main Results:

Sucrose acts as a signaling molecule regulating translation of S1 class bZIP genes through uORFs.
uORF2 in bZIP11 encodes the conserved SC-peptide, crucial for sucrose-dependent translational control.
bZIP11 proteins are targeted by SnRK1 kinases, linking energy status to translational regulation and ribosomal biogenesis.

Conclusions:

Plants employ translational control for optimizing growth under stress and energy deprivation.
Sucrose signaling, mediated by bZIP11 and its SC-peptide, is a key mechanism for adapting gene expression to metabolic status.