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Related Experiment Video

Updated: Jun 23, 2026

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

Universal sample preparation method for proteome analysis.

Jacek R Wiśniewski1, Alexandre Zougman, Nagarjuna Nagaraj

  • 1Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany. jwisniew@biochem.mpg.de

Nature Methods
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

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Filter-aided sample preparation (FASP) enhances mass spectrometry proteomics by combining in-gel and in-solution digestion. This method yields pure peptides for deeper proteome coverage and single-run analyses.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry-based proteomics is crucial for biological research.
  • Existing methods like in-gel and in-solution digestion have limitations.
  • Efficient sample preparation is key to achieving deep proteome coverage.

Purpose of the Study:

  • To introduce a novel sample preparation technique for mass spectrometry-based proteomics.
  • To combine the benefits of in-gel and in-solution digestion methods.
  • To improve the depth and efficiency of proteome analysis.

Main Methods:

  • Developed filter-aided sample preparation (FASP).
  • Completely solubilized the proteome using sodium dodecyl sulfate.
  • Exchanged solubilizing agents with urea on a filtration device.

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Published on: November 15, 2017

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
11:12

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Published on: February 7, 2022

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

  • Performed on-filter digestion and peptide elution.

    • Main Results:

    • Achieved pure peptides after on-filter digestion.
    • Enabled single-run analyses of complex samples like organelles.
    • Demonstrated unprecedented depth of proteome coverage.
    • FASP combines advantages of in-gel and in-solution digestion.

    Conclusions:

    • Filter-aided sample preparation (FASP) is an effective method for mass spectrometry proteomics.
    • FASP simplifies sample preparation and enhances proteome coverage.
    • The technique facilitates in-depth analysis of complex biological samples.