Universal sample preparation method for proteome analysis
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Updated: Jun 23, 2026
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Published on: February 7, 2022
Jacek R Wiśniewski1, Alexandre Zougman, Nagarjuna Nagaraj
1Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany. jwisniew@biochem.mpg.de
Abstract:
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
