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Updated: Jun 23, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
[Potential application of human amniotic mesenchymal cells for seeding cells in bone tissue engineering]
Fang Mei1, Jun-min Tang, Jin-yan Zhong
1Department of Anatomy and Histoembryology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
Objective:
To investigate the potential of human amniotic mesenchymal cells (hAMC) serving as seeding cells in bone tissue engineering.
Methods:
hAMC were isolated and cultured. The third passage of hAMC was cultured in osteogenic induced media [DMEM supplemented with 10% (v/v) FBS, 0.1 mumol/L dexamethasone, 50 mg/L ascorbic acid and 10 mmol/L beta-glycerophosphate] for one week. Calcified nodules were shown by alizarin red staining and counted under light microscope. Immunofluorescence cytochemical staining was used to detect collagen I (COL I) and alkaline phosphatase (ALP). Expression of FasL was examined in the amnion and hAMC by immunohistochemistry or immunocytochemistry.
Results:
After osteoblast differentiation, calcified nodules were formed, on the average 18 per well. hAMC in calcified nodules showed positive expression of COL I and ALP. FasL was detected positive both in cells contained in amnion and in hAMC.
Conclusion:
hAMC are potential ideal candidates for seeding cells in bone tissue engineering.
