Jove
Visualize
Contact Us

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Online affinity micro free-flow electrophoresis for the continuous monitoring of insulin <i>via</i> a competitive immunoassay.

Lab on a chip·2026
Same author

Identifying and minimizing primary sources of temporal broadening in online affinity micro free-flow electrophoresis.

The Analyst·2025
Same author

Assessing Surface Adsorption in Cyclic Olefin Copolymer Microfluidic Devices Using Two-Dimensional Nano Liquid Chromatography-Micro Free Flow Electrophoresis Separations.

Analytical chemistry·2023
Same author

Fabrication of µFFE Devices in COC via Hot Embossing with a 3D-Printed Master Mold.

Micromachines·2023
Same author

Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free-flow electrophoresis devices.

Electrophoresis·2019
Same author

Cytoplasmic nucleic acid-based XNAs directly enhance live cardiac cell function by a Ca<sup>2+</sup> cycling-independent mechanism via the sarcomere.

Journal of molecular and cellular cardiology·2019
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Isolating aptamers using capillary electrophoresis-SELEX (CE-SELEX).

Renee K Mosing1, Michael T Bowser

  • 1University of Minnesota, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary

Systematic evolution of ligands by exponential enrichment (SELEX) can be significantly accelerated using capillary electrophoresis (CE). CE-SELEX isolates high-affinity aptamers in just 2-4 rounds, reducing process time from weeks to days.

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Related Experiment Videos

Last Updated: Jun 23, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • SELEX is a standard method for aptamer discovery.
  • Traditional SELEX requires 10-15 cycles, which is time-consuming.
  • Aptamers are crucial for molecular targets due to their high affinity and selectivity.

Purpose of the Study:

  • To improve the efficiency of SELEX.
  • To reduce the time required for aptamer isolation.
  • To demonstrate the utility of capillary electrophoresis in SELEX.

Main Methods:

  • Incorporation of capillary electrophoresis (CE) as an enrichment step in SELEX.
  • Performing 2-4 rounds of CE-SELEX.
  • Standard SELEX procedures including PCR amplification and nucleic acid purification.

Main Results:

  • CE-SELEX significantly improved the efficiency of aptamer selection.
  • High-affinity aptamers were isolated in only 2-4 rounds.
  • The overall process time was reduced from several weeks to a few days.

Conclusions:

  • CE-SELEX is a highly efficient method for aptamer isolation.
  • This method drastically reduces the time and resources needed for aptamer development.
  • CE-SELEX offers a faster alternative for discovering DNA or RNA aptamers.