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Updated: Jun 23, 2026

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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Published on: August 9, 2022
In vivo imaging of oligonucleotidic aptamers
Bertrand Tavitian1, Frédéric Ducongé, Raphaël Boisgard
1Laboratoire d Imagerie Moléculaire Expérimentale, Service hospitalier Frédéric joliot, Intitut d Imagerie Biomédicale, Orsay, France.
Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
Abstract:
In this chapter we present the methods developed in our laboratory for in vivo imaging of oligonucleotidic aptamers. These methods relate to (i) the labelling of aptamers with fluorine-18, a positron emitter, (ii) Positron Emission Tomography imaging of laboratory animals with [(18)F]aptamers and (iii) labelling with fluorescent dyes and optical imaging of aptamers in mice.
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In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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