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Updated: Jun 23, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Locked nucleic acid aptamers.

Jan Barciszewski1, Michael Medgaard, Troels Koch

  • 1Institute of Bioorganic Chemistry of the Polish Academy of Sciences, , Poznan, Poland.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary
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Locked nucleic acid aptamers (LNA-Aps) offer a stable, low-toxicity alternative to traditional therapeutics. These engineered molecules bind targets with high affinity, enabling diverse diagnostic and therapeutic applications.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Discovery

Background:

  • Aptamer technology, introduced in the 1990s, identifies ligands for various targets.
  • Aptamers serve as inhibitory molecules for functional interference in biological systems.
  • Applications range from diagnostics to therapeutics.

Purpose of the Study:

  • To introduce Locked Nucleic Acid Aptamers (LNA-Aps) as an advanced aptamer technology.
  • To highlight the unique structural and binding properties of LNA-Aps.
  • To emphasize their potential as therapeutic agents.

Main Methods:

  • In vitro evolution of oligonucleotides containing Locked Nucleic Acid (LNA) monomers.
  • Engineering LNA-Aps for high-affinity and specific binding to target ligands.

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  • Characterization of LNA-Aps for stability, toxicity, and immunogenicity.

    • Main Results:

    • LNA-Aps exhibit high affinity and specificity for target ligands.
    • These aptamers possess enhanced stability compared to traditional oligonucleotides.
    • LNA-Aps demonstrate low toxicity and immunogenicity.

    Conclusions:

    • LNA-Aps represent a promising advancement in aptamer technology.
    • Their stability, specificity, and safety profile make them attractive therapeutic candidates.
    • LNA-Aps offer a viable alternative to antibody- and small-molecule-based therapeutics.