Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flow-dependent NRF2 dysfunction via KEAP1/WDR23 dual repression contributes to endothelial oxidative damage in venous disease.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Altered venous flow drives endothelial to mesenchymal transition in varicose veins by suppressing PIEZO1-KLF2 signaling.

Cellular and molecular life sciences : CMLS·2025
Same author

Mechanoresponsive ETS1 causes endothelial dysfunction and arterialization in varicose veins via NOTCH4/DLL4 signaling.

European journal of cell biology·2024
Same author

Oscillatory shear stress modulates Notch-mediated endothelial mesenchymal plasticity in cerebral arteriovenous malformations.

Cellular & molecular biology letters·2023
Same author

Molecular Biomarkers and Drug Targets in Brain Arteriovenous and Cavernous Malformations: Where Are We?

Stroke·2021
Same author

Heat shock proteins-driven stress granule dynamics: yet another avenue for cell survival.

Apoptosis : an international journal on programmed cell death·2021
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Dot-immunobinding assay.

S Sumi1, A Mathai, V V Radhakrishnan

  • 1Department of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST), Thiruvananthapuram, 695 011, Kerala, India.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

Dot-immunobinding assay (Dot-Iba) offers a simple, rapid method for diagnosing diseases like tuberculous meningitis. This highly specific assay is ideal for resource-limited settings, showing no false positives.

More Related Videos

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
08:15

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Published on: December 9, 2022

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens
07:39

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jun 23, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
08:15

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Published on: December 9, 2022

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens
07:39

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens

Published on: June 23, 2022

Area of Science:

  • Immunodiagnostics
  • Medical laboratory technology

Background:

  • The Dot-immunobinding assay (Dot-Iba) is a straightforward and reproducible immunodiagnostic technique.
  • It involves immobilizing antibodies or antigens onto nitrocellulose membrane (NCM) discs for sample analysis.

Purpose of the Study:

  • To evaluate the efficacy and applicability of the Dot-immunobinding assay (Dot-Iba) as a diagnostic tool.
  • To assess its suitability for resource-limited laboratory settings.

Main Methods:

  • Antigen or antibody is dotted onto nitrocellulose membrane (NCM) discs.
  • Diagnostic samples are incubated on the discs, followed by detection with enzyme-conjugated antiglobulins and substrate.
  • Positive results are indicated by a purple-pink colored, insoluble substrate product.

Main Results:

  • The Dot-Iba assay demonstrated an overall sensitivity of 60% for tuberculous meningitis diagnosis.
  • The assay exhibited high specificity, with no false positive results observed.
  • It is particularly effective for diagnosing paucibacillary diseases, including extrapulmonary tuberculosis.

Conclusions:

  • Dot-immunobinding assay (Dot-Iba) is a rapid, simple, and highly specific immunoassay.
  • It is well-suited for diagnosing paucibacillary diseases and is particularly valuable for laboratories in developing countries with limited resources.