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Updated: Jun 23, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
Published on: September 15, 2016
Sequential use of immunoblots for characterization of autoantibody specificities
Holger Bartsch1, Michael Bachmann
1Institute Immunology, MTZ, Medical Faculty, Carl Gustav Carus University TU Dresden, Fiedlerstrasse 42, D-01307, Dresden, Germany.
Abstract:
Sera of patients with systemic autoimmune diseases frequently contain autoantibodies to nuclear autoantigens. Immunoblotting of recombinant and native autoantigens is a commonly used technique for the identification and characterization of autoantibody specificities. Here, we describe an easy procedure that facilitates the comparison of antibody specificities by reusing the same immunoblot at least three times in order to detect an abundantly expressed autoantigen in total cellular extracts.
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