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Updated: Jun 23, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Zebrafish spotted-microarray for genome-wide expression profiling experiments: data acquisition and analysis.

Siew Hong Lam1, R Krishna Murthy Karuturi, Zhiyuan Gong

  • 1Department of Biological Sciences, S3-Level 5, 14 Science Drive 4, National University of Singapore, Singapore, 117543.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
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This chapter details the computational "dry-lab procedures" for expression microarray analysis, focusing on acquiring and transforming fluorescent signals into biologically significant data for zebrafish expression profiling experiments.

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Expression microarray experiments generate large datasets of RNA abundance.
  • Post-hybridization procedures, termed "dry-lab procedures," are crucial for data interpretation.
  • These computational steps are essential for extracting biological meaning from raw experimental data.

Purpose of the Study:

  • To describe the computational steps for acquiring and analyzing expression microarray data.
  • To provide guidance on data normalization, processing, and analysis strategies.
  • To ensure effective downstream data handling and interpretation in zebrafish expression profiling.

Main Methods:

  • Image and data acquisition from fluorescent signals.
  • Data normalization and processing techniques.

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Last Updated: Jun 23, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
09:38

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

Published on: January 24, 2025

  • Primary and secondary data analysis methodologies.

    • Main Results:

    • Acquisition of fluorescent signals representing RNA abundance.
    • Transformation of signals into meaningful biological data.
    • Application of these methods to zebrafish expression profiling.

    Conclusions:

    • A strategic approach to data analysis planning is vital before wet-lab procedures.
    • Proper dry-lab procedures are essential for reliable interpretation of microarray data.
    • This chapter offers practical guidance for researchers conducting zebrafish expression profiling.