Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
09:27

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Published on: January 17, 2014

Using fluorescent proteins to study poxvirus morphogenesis.

Brian M Ward1

  • 1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RSV-NTHi Co-Infection Skews Host Immunity by Suppressing Type I IFN Responses and Enhancing Pro-Inflammatory Responses.

Pathogens (Basel, Switzerland)·2026
Same author

VopX, a novel <i>Vibrio cholerae</i> T3SS effector, modulates host actin dynamics.

mBio·2025
Same author

A Quantitative Real-Time PCR Assay for Measuring Poxvirus Replication and Cell Binding.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Deciphering genetic factors contributing to enhanced resistance against Cercospora leaf blight in soybean (<i>Glycine max</i> L.) using GWAS analysis.

Frontiers in genetics·2024
Same author

The Antiviral Effect of Berdazimer Sodium on Molluscum Contagiosum Virus Using a Novel In Vitro Methodology.

Viruses·2023
Same author

Characterization of QoI-Fungicide Resistance in <i>Cercospora</i> Isolates Associated with Cercospora Leaf Blight of Soybean from the Southern United States.

Plant disease·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Fluorescent protein fusions enable real-time tracking of viral protein movement within cells. This technique visualizes poxvirus assembly and helps map gene functions by observing altered fluorescent patterns.

Area of Science:

  • Microbiology
  • Cell Biology
  • Virology

Background:

  • Fluorescent protein (FP) fusions visualize protein localization and trafficking in real-time.
  • FP-fusion patterns serve as visual markers for subcellular processes.
  • FP-fusions are applied to study poxvirus morphogenesis.

Purpose of the Study:

  • To utilize FP-fusions for studying poxvirus morphogenesis at the subcellular level.
  • To establish a method for mapping functional domains of viral proteins using FP-fusions and complementation assays.
  • To describe procedures for cell infection, transfection, and microscopy-based analysis for functional domain mapping.

Main Methods:

  • Constructing recombinant poxviruses expressing FP-fusions with viral proteins.
  • Generating viral mutants with deleted genes involved in morphogenesis or egress.

More Related Videos

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
09:25

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Published on: May 24, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
09:27

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Published on: January 17, 2014

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
09:25

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Published on: May 24, 2020

  • Performing in-trans complementation assays in infected and transfected cells.
  • Analyzing subcellular FP patterns using live or static microscopy.

    • Main Results:

    • FP-fusions with viral core or extracellular proteins provide a visual readout of normal poxvirus morphogenesis.
    • Deletion of key viral genes results in aberrant FP patterns, indicating roles in morphogenesis or egress.
    • Complementation assays combined with FP pattern analysis allow for functional domain mapping of deleted viral proteins.

    Conclusions:

    • FP-fusions are a powerful tool for visualizing and analyzing poxvirus morphogenesis.
    • This methodology enables the functional dissection of viral genes and their roles in the viral life cycle.
    • The described techniques facilitate the study of viral protein function and host-pathogen interactions.